A Method for Reconstruction of Severely Damaged Sciatic nerve using non-Autologous Wharton's Jelly Mesenchymal Stem Cells and Chitosan/Poly ethylene oxide as a Synthetic Scaffold: Static sciatic index (SSI)Name : Dr. Mehrnaz Moattari
Affliation : Professor
University : Kharazmi university
Country : Iran
There have been attempts to alter the status of severest sciatic nerve injury in different investigations, but none of which have reliably altered the outcome. Some studies use stem cells (SCs) that produced widely differing results. We hereby add our experience in Neuroscience research center of a surgical reconstruction of the damaged sciatic nerve using a mixture of SCs and Chitosan/Poly ethylene Oxide (Cs/PEO) as a synthetic scaffold.
Materials and Methods:
Thirty rats of severely damaged sciatic nerve have been operated for end-to-end suture and reconstruction of the sciatic nerve using SCs and Chitosan/poly ethylene oxide (Cs/PEO). Cs/PEO serves to maintain the position of the SCs. Following PNI or sham surgery, Using a freely available image-editing program (Axio Vision, Zeiss, Jena, Germany), the distance between toe 1 and 5 (toe spread TS) as well as 2 and 4 (intermediate toe spread ITS) was measured for the lesioned (LTS; LITS) and non-lesioned (NTS; NITS) hind paws. The SSI was then calculated using the following equations (TSF ¼ toe spread factor; ITSF ¼ intermediate toe spread factor): TSF ¼ (LTS _ NTS)/NTS; ITSF ¼ (LITS _ NITS) NITS; SSI ¼ (108.44 * TSF) (31.85 * ITSF) _ 5.49.
Following transection injury, the post-surgery performances of the different experimental groups revealed no significant differences, also when comparing the reconstruction of the nerve using a mixture of SCs and Chitosan/Poly ethylene Oxide (Cs/PEO) as a synthetic scaffold (p < 0.05).
Using a mixture of SCs and Chitosan/Poly ethylene Oxide (Cs/PEO) as a synthetic scaffold may play a role in restoring sciatic nerve functions.
Keywords: Synthetic scaffold, Non-autologous stem cells, Sciatic nerve injury